mouse anti phospho egfr Search Results


mouse anti-human phospho-egfr (tyr-1068  (Becton Dickinson)
90
Becton Dickinson mouse anti-human phospho-egfr (tyr-1068
Mouse Anti Human Phospho Egfr (Tyr 1068, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+phospho+egfr/mouse+anti+human+phospho+egfr++tyr+1068/pmc03318723-60-0-28
Average 90 stars, based on 1 article reviews
mouse anti-human phospho-egfr (tyr-1068 - by Bioz Stars, 2026-09
90/100 stars
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mouse monoclonal (mab) anti-phospho-egfr (activated form  (Becton Dickinson)
90
Becton Dickinson mouse monoclonal (mab) anti-phospho-egfr (activated form
Mutant EGF receptors colocalize <t>with</t> <t>Src</t> in NSCLCcell lines . Cells were growth factor-deprived in 0.1% FBS-containing growth medium for 48 hr and then either left unstimulated (- EGF) or stimulated (+ EGF) with 100 ng/ml of EGF for 10 min. Cells were fixed, permeabilized, and immunostained with (A) <t>anti-EGFR</t> antibody 528 (green) followed by anti-Src antibody SRC2 (red) staining or (B) anti-phospho-EGFR antibody (red) followed by anti-phospho-Src antibody (green). Images were acquired under a confocal microscope at the medial plane. Arrows indicate EGFR and Src colocalization. Bars represent 20 μm. (C) Colocalization coefficients for the green channel (EGFR) from (A), and (D) colocalization coefficients for the red channel (pEGFR) from (B) were obtained using LSM510 Image Examiner software and normalized to the colocalization coefficient from unstimulated (- EGF) H1666 cells.
Mouse Monoclonal (Mab) Anti Phospho Egfr (Activated Form, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+phospho+egfr/mouse+monoclonal++mab++anti+phospho+egfr++activated+form/pmc02790444-167-40-48
Average 90 stars, based on 1 article reviews
mouse monoclonal (mab) anti-phospho-egfr (activated form - by Bioz Stars, 2026-09
90/100 stars
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anti-phospho-egfr(y1173)/her2(y1248) mouse igg  (Abfrontier ltd)
90
Abfrontier ltd anti-phospho-egfr(y1173)/her2(y1248) mouse igg
Mutant EGF receptors colocalize <t>with</t> <t>Src</t> in NSCLCcell lines . Cells were growth factor-deprived in 0.1% FBS-containing growth medium for 48 hr and then either left unstimulated (- EGF) or stimulated (+ EGF) with 100 ng/ml of EGF for 10 min. Cells were fixed, permeabilized, and immunostained with (A) <t>anti-EGFR</t> antibody 528 (green) followed by anti-Src antibody SRC2 (red) staining or (B) anti-phospho-EGFR antibody (red) followed by anti-phospho-Src antibody (green). Images were acquired under a confocal microscope at the medial plane. Arrows indicate EGFR and Src colocalization. Bars represent 20 μm. (C) Colocalization coefficients for the green channel (EGFR) from (A), and (D) colocalization coefficients for the red channel (pEGFR) from (B) were obtained using LSM510 Image Examiner software and normalized to the colocalization coefficient from unstimulated (- EGF) H1666 cells.
Anti Phospho Egfr(y1173)/Her2(y1248) Mouse Igg, supplied by Abfrontier ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+phospho+egfr/anti+phospho+egfr+y1173++her2+y1248++mouse+igg/pm19716804-34-32-35
Average 90 stars, based on 1 article reviews
anti-phospho-egfr(y1173)/her2(y1248) mouse igg - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Mutant EGF receptors colocalize with Src in NSCLCcell lines . Cells were growth factor-deprived in 0.1% FBS-containing growth medium for 48 hr and then either left unstimulated (- EGF) or stimulated (+ EGF) with 100 ng/ml of EGF for 10 min. Cells were fixed, permeabilized, and immunostained with (A) anti-EGFR antibody 528 (green) followed by anti-Src antibody SRC2 (red) staining or (B) anti-phospho-EGFR antibody (red) followed by anti-phospho-Src antibody (green). Images were acquired under a confocal microscope at the medial plane. Arrows indicate EGFR and Src colocalization. Bars represent 20 μm. (C) Colocalization coefficients for the green channel (EGFR) from (A), and (D) colocalization coefficients for the red channel (pEGFR) from (B) were obtained using LSM510 Image Examiner software and normalized to the colocalization coefficient from unstimulated (- EGF) H1666 cells.

Journal: BMC Cell Biology

Article Title: Aberrant trafficking of NSCLC-associated EGFR mutants through the endocytic recycling pathway promotes interaction with Src @

doi: 10.1186/1471-2121-10-84

Figure Lengend Snippet: Mutant EGF receptors colocalize with Src in NSCLCcell lines . Cells were growth factor-deprived in 0.1% FBS-containing growth medium for 48 hr and then either left unstimulated (- EGF) or stimulated (+ EGF) with 100 ng/ml of EGF for 10 min. Cells were fixed, permeabilized, and immunostained with (A) anti-EGFR antibody 528 (green) followed by anti-Src antibody SRC2 (red) staining or (B) anti-phospho-EGFR antibody (red) followed by anti-phospho-Src antibody (green). Images were acquired under a confocal microscope at the medial plane. Arrows indicate EGFR and Src colocalization. Bars represent 20 μm. (C) Colocalization coefficients for the green channel (EGFR) from (A), and (D) colocalization coefficients for the red channel (pEGFR) from (B) were obtained using LSM510 Image Examiner software and normalized to the colocalization coefficient from unstimulated (- EGF) H1666 cells.

Article Snippet: The following antibodies were obtained from commercial sources: rabbit polyclonal (pAb) anti-EGFR (1005), pAb anti-phospho-AKT (pAKT1/2/3) (Ser 473), pAb anti-phospho-Erk 1/2 (Thr 202/Tyr 204), pAb anti-Erk1 (K-23), and pAb anti-Src (SRC 2) were from Santa Cruz Biotechnology (Santa Cruz, CA); mouse monoclonal (mAb) anti-phospho-EGFR (activated form) was from BD Biosciences (San Jose, CA); pAb anti-phospho-Src (Tyr416), pAb anti-phospho-EGFR (Tyr1173), pAb anti-STAT3, Rabbit monoclonal anti-phospho-STAT3 (Tyr705), and pAb anti-phospho-EGFR (Tyr845) were from Cell Signaling Technology (Danvers, MA); mAb anti-β actin (Clone AC-15) was from Sigma-Aldrich (St Louis, MO); mAb anti-LAMP1 (H4A3) was from Developmental Studies Hybridoma Bank (Iowa City, IA); mAb anti-EGFR (clone 528; ATCC) was Protein G purified from hybridoma supernatants.

Techniques: Mutagenesis, Staining, Microscopy, Software

Monensin treatment enhances mutant EGFR-Src colocalization in endocytic vesicles . (A) Cells were either growth factor-deprived or left in regular growth medium for 48 hr and incubated with DMSO or 10 μM monensin for 3 hr. Cells were fixed, permeabilized, and immunostained with anti-EGFR antibody 528 (green) followed by anti-Src antibody SRC2 (red) staining. Images were acquired under a confocal microscope at the medial plane. Bars represent 20 μm. (B) Colocalization coefficients for the red channel (anti-Src staining) were obtained using LSM510 Image Examiner software and normalized to the colocalization coefficient from DMSO controls.

Journal: BMC Cell Biology

Article Title: Aberrant trafficking of NSCLC-associated EGFR mutants through the endocytic recycling pathway promotes interaction with Src @

doi: 10.1186/1471-2121-10-84

Figure Lengend Snippet: Monensin treatment enhances mutant EGFR-Src colocalization in endocytic vesicles . (A) Cells were either growth factor-deprived or left in regular growth medium for 48 hr and incubated with DMSO or 10 μM monensin for 3 hr. Cells were fixed, permeabilized, and immunostained with anti-EGFR antibody 528 (green) followed by anti-Src antibody SRC2 (red) staining. Images were acquired under a confocal microscope at the medial plane. Bars represent 20 μm. (B) Colocalization coefficients for the red channel (anti-Src staining) were obtained using LSM510 Image Examiner software and normalized to the colocalization coefficient from DMSO controls.

Article Snippet: The following antibodies were obtained from commercial sources: rabbit polyclonal (pAb) anti-EGFR (1005), pAb anti-phospho-AKT (pAKT1/2/3) (Ser 473), pAb anti-phospho-Erk 1/2 (Thr 202/Tyr 204), pAb anti-Erk1 (K-23), and pAb anti-Src (SRC 2) were from Santa Cruz Biotechnology (Santa Cruz, CA); mouse monoclonal (mAb) anti-phospho-EGFR (activated form) was from BD Biosciences (San Jose, CA); pAb anti-phospho-Src (Tyr416), pAb anti-phospho-EGFR (Tyr1173), pAb anti-STAT3, Rabbit monoclonal anti-phospho-STAT3 (Tyr705), and pAb anti-phospho-EGFR (Tyr845) were from Cell Signaling Technology (Danvers, MA); mAb anti-β actin (Clone AC-15) was from Sigma-Aldrich (St Louis, MO); mAb anti-LAMP1 (H4A3) was from Developmental Studies Hybridoma Bank (Iowa City, IA); mAb anti-EGFR (clone 528; ATCC) was Protein G purified from hybridoma supernatants.

Techniques: Mutagenesis, Incubation, Staining, Microscopy, Software

Monensin treatment enhances the mutant EGFR-Src association . Cells were growth factor-deprived for 48 hr and incubated with DMSO (D) or 10 μM monensin (M) for 3 hr. 1 mg aliquots of cell lysate proteins were immunoprecipitated with anti-EGFR antibody 528 and resolved together with 50 μg aliquots of whole cell lysates followed by immunoblotting with antibodies against the indicated proteins. The levels of EGFR and Src bands on blots were quantified using densitometry and immunoprecipitated Src relative to immunoprecipitated EGFR levels (Src/EGFR) were determined using the ImageJ software. Src/EGFR ratios in monensin-treated cells versus DMSO-treated cells are noted.

Journal: BMC Cell Biology

Article Title: Aberrant trafficking of NSCLC-associated EGFR mutants through the endocytic recycling pathway promotes interaction with Src @

doi: 10.1186/1471-2121-10-84

Figure Lengend Snippet: Monensin treatment enhances the mutant EGFR-Src association . Cells were growth factor-deprived for 48 hr and incubated with DMSO (D) or 10 μM monensin (M) for 3 hr. 1 mg aliquots of cell lysate proteins were immunoprecipitated with anti-EGFR antibody 528 and resolved together with 50 μg aliquots of whole cell lysates followed by immunoblotting with antibodies against the indicated proteins. The levels of EGFR and Src bands on blots were quantified using densitometry and immunoprecipitated Src relative to immunoprecipitated EGFR levels (Src/EGFR) were determined using the ImageJ software. Src/EGFR ratios in monensin-treated cells versus DMSO-treated cells are noted.

Article Snippet: The following antibodies were obtained from commercial sources: rabbit polyclonal (pAb) anti-EGFR (1005), pAb anti-phospho-AKT (pAKT1/2/3) (Ser 473), pAb anti-phospho-Erk 1/2 (Thr 202/Tyr 204), pAb anti-Erk1 (K-23), and pAb anti-Src (SRC 2) were from Santa Cruz Biotechnology (Santa Cruz, CA); mouse monoclonal (mAb) anti-phospho-EGFR (activated form) was from BD Biosciences (San Jose, CA); pAb anti-phospho-Src (Tyr416), pAb anti-phospho-EGFR (Tyr1173), pAb anti-STAT3, Rabbit monoclonal anti-phospho-STAT3 (Tyr705), and pAb anti-phospho-EGFR (Tyr845) were from Cell Signaling Technology (Danvers, MA); mAb anti-β actin (Clone AC-15) was from Sigma-Aldrich (St Louis, MO); mAb anti-LAMP1 (H4A3) was from Developmental Studies Hybridoma Bank (Iowa City, IA); mAb anti-EGFR (clone 528; ATCC) was Protein G purified from hybridoma supernatants.

Techniques: Mutagenesis, Incubation, Immunoprecipitation, Western Blot, Software